Academic Online Course in Western Blotting

Immerse yourself in the world of protein extraction through our exceptional academic course, tailored for students, researchers, and healthcare professionals in the field of life sciences.

Course outlines:

Introduction to Western Blotting

Principles and fundamentals of Western blotting

Applications and significance of Western blot analysis

Comparison with other protein detection methods

Sample Preparation and Protein Separation

Sample collection and preparation for Western blotting

Protein extraction methods and sample solubilization

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for protein separation

Transfer and Membrane Blocking

Transfer methods (wet, semi-dry) for protein transfer onto membranes

Membrane selection and preparation

Blocking techniques to prevent nonspecific binding

Antibody Selection and Validation

Choosing primary and secondary antibodies for target protein detection

Antibody validation methods and considerations

Troubleshooting antibody-related issues

Immunodetection and Signal Development

Immunodetection methods (chemiluminescence, fluorescence)

Signal development and substrate selection

Optimizing antibody concentrations and incubation conditions

Data Analysis and Quantification

Image acquisition and analysis software for Western blot data

Quantification methods for protein band intensity

Statistical analysis and interpretation of Western blot results

Troubleshooting and Optimization

Common issues and challenges in Western blotting

Troubleshooting strategies for artifact reduction

Optimization of experimental conditions for improved results

Advanced Applications and Techniques

Multiplex Western blotting for simultaneous protein detection

Phosphorylation-specific Western blotting techniques

Quantitative Western blotting approaches (e.g., densitometry, standard curves)

Practical Hands-on Training

Laboratory sessions to practice Western blotting techniques

Sample preparation, gel electrophoresis, and membrane transfer experiments

Antibody incubation, signal development, and image analysis

Course Duration:

10 hours

Our course welcomes students and professionals with a background in life sciences or related disciplines.

Contact Information:

For registration, inquiries, or further information, please don’t hesitate to reach out to our coordinator (Dr. B. Gunay) via WhatsApp at [+90 538 455 80 29]. Additionally, feel free to leave a reply and post your comment in the box below. Our dedicated team will be delighted to assist you promptly with any queries you may have.

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