Academic Online Course in Protein Extraction

Immerse yourself in the world of protein extraction through our exceptional academic course, tailored for students, researchers, and healthcare professionals in the field of life sciences.

Course outlines:

Introduction to Protein Extraction

Importance of protein extraction in biological research

Different protein extraction methods and their principles

Factors influencing protein extraction efficiency

Sample Preparation and Lysis

Sample collection and preparation techniques

Cell lysis methods for different sample types (cells, tissues, organisms)

Optimization of lysis conditions for protein extraction

Solubilization of Proteins

Protein solubilization strategies and buffers

Use of detergents and chaotropic agents

Minimizing protein degradation during solubilization

Extraction of Membrane Proteins

Techniques for extraction of membrane-bound proteins

Removal of lipids and contaminants from membrane protein samples

Solubilization of integral and peripheral membrane proteins

Fractionation and Subcellular Localization

Subcellular fractionation techniques for protein extraction

Isolation of specific organelles and subcellular compartments

Enrichment of target proteins using fractionation methods

Protein Purification

Overview of protein purification methods

Principles and applications of chromatography techniques

Affinity purification, ion exchange, size exclusion chromatography

Quantification and Analysis

Protein quantification methods (Bradford, BCA, spectrophotometry)

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)

Western blotting and immunodetection techniques

Quality Control and Troubleshooting

Assessing protein quality and integrity

Troubleshooting common issues in protein extraction

Ensuring reproducibility and reliability in protein analysis

Practical Hands-on Training

Laboratory sessions to practice protein extraction techniques

Sample preparation, lysis, and solubilization experiments

Protein quantification and analysis using various methods

Course Duration:

10 hours

Our course welcomes students and professionals with a background in life sciences or related disciplines.

Contact Information:

For registration, inquiries, or further information, please don’t hesitate to reach out to our coordinator (Dr. B. Gunay) via WhatsApp at [+90 538 455 80 29]. Additionally, feel free to leave a reply and post your comment in the box below. Our dedicated team will be delighted to assist you promptly with any queries you may have.

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